A new set of assays for the discovery of aminoacyl-tRNA synthetase inhibitors

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• 作者：Adé ; laï ; de Saint-Lé ; ger^a ; Lluí ; s Ribas de Pouplana^a ; ^b ; ^c ; ^{lluis.ribas@irbbarcelona.org}
• 关键词：ARS ; aminoacyl-tRNA synthetases ; Cpd ; compound ; HTS ; high throughput screening ; IC50 ; half maximal inhibitory concentration ; Km ; Michaelis constant ; LRS ; leucyl-tRNA synthetase ; MIC ; minimum inhibitory concentration ; OM ; Omnia Molecular ; RLU ; relative light unit ; RRS ; arginyl-tRNA synthetase ; RT ; room temperature ; SRS ; seryl-tRNA synthetase ; SD ; standard deviation ; tRNA ; aminoacylate transfer RNA ; YRS ; tyrosyl-tRNA synthetase ; WRS ; tryptophanyl-tRNA synthetase ; Wt ; wild-type
• 刊名：Methods
• 出版年：2017
• 出版时间：15 January 2017
• 年：2017
• 卷：113
• 期：Complete
• 页码：34-45
• 全文大小：4871 K
• 卷排序：113

文摘

Methods to monitor ARS activity: in vitro, in human cells and in bacteria. Utilization of tRNA suppressors for drug discovery in human cells. Use of complementation assays to detect species-specific ARS inhibition. Monitoring ARS activity via ATP consumption and bioluminescence.