Conformation-sensitive antibody-based point-of-care immunosensor for serum Ca2+ using two-dimensional sequential binding reactions
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- 作者：Ji-Na Parka ; Sung-Ho Paeka ; Dong-Hyung Kima ; Sung-Min Seoa ; Guei-Sam Lima ; b ; Ju-Hee Kanga ; Sung-Pil Paekc ; Il-Hoon Chod ; Se-Hwan Paeka ; e ; shpaek@korea.ac.kr" class="auth_mail" title="E-mail the corresponding author
- 关键词：Conformational change of calcium-binding protein ; Conformation-specific antibody ; Point-of-care immunosensor for Ca2+ ; Highly resolved dynamic range ; Smartphone-based immunosensor
- 刊名：Biosensors and Bioelectronics
- 出版年：2016
- 出版时间：15 November 2016
- 年：2016
- 卷：85
- 期：Complete
- 页码：611-617
- 全文大小：1273 K
文摘
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A rapid immunosensor was developed to assess the homeostasis of Ca2+ metabolism.
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CBP undergone conformation change upon Ca2+ binding was discriminated using antibody.
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2-D chromatography was used to analyze serum Ca2+ in a clinical range of 0.25–2.5 mM.
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This inspired us to further investigate a dual-immunoassay system for Ca2+ and PTH.