Asymmetric whole-cell bioreduction of (R)-carvone by recombinant Escherichia coli with in situ substrate supply and product removal

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• 作者：Kathrin Castiglione ; ^{k.castiglione@lrz.tum.de} ; Yilei Fu ; Ingmar Polte ; Simeon Leupold ; Andrea Meo ; Dirk Weuster-Botz
• 关键词：Adsorbent resin ; Biotransformation ; (R)-Carvone ; Ene-reductase ; Formate dehydrogenase ; Ionic liquid
• 刊名：Biochemical Engineering Journal
• 出版年：2017
• 出版时间：15 January 2017
• 年：2017
• 卷：117
• 期：part_PA
• 页码：102-111
• 全文大小：1368 K
• 卷排序：117

文摘

(R)-carvone was reduced to (2R,5R)-dihydrocarvone by recombinant Escherichia coli. No alcohols were formed as side-products in biotransformations up to the L-scale. In situ substrate feeding and product removal (SFPR) improved the bioreduction. Using ionic liquids or adsorbent resins for SFPR led to high conversions (>85%). Concomitantly, the product was gained in high diastereomeric excess (up to 99.2%).