Engineering foot-and-mouth disease virus serotype O IND R2/1975 for one-step purification by immobilized metal affinity chromatography
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- 作者：Jitendra K. Biswal ; jkubiswal@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Punam Bisht ; Saravanan Subramaniam ; Rajeev Ranjan ; Gaurav K. Sharma ; Bramhadev Pattnaik ; pattnaikb@gmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：FMD virus ; Affinity chromatography ; His6-tagged FMDV ; Reverse genetics ; FMDV purification
- 刊名：Biologicals
- 出版年：2015
- 出版时间：September 2015
- 年：2015
- 卷：43
- 期：5
- 页码：390-398
- 全文大小：1206 K
文摘
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His6-tagged FMDV was constructed using reverse genetics approach.
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His6-tag was introduced at the previously reported site in the VP1 G-H loop of FMDV.
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Display of His6-tag endowed the virus with an affinity for immobilized nickel ions.
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The tagged-FMDV would be useful for purification by IMAC.
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The tagged virus retains its functionality and infectivity similar to the non-tagged virus.