• 作者：Shafiqul Alam Sarker^a ; Shamima Sultana^a ; Gloria Reuteler^b ; Deborah Moine^c ; Patrick Descombes^c ; Florence Charton^b ; Gilles Bourdin^b ; Shawna McCallin^b ; Catherine Ngom-Bru^b ; Tara Neville^b ; Mahmuda Akter^a ; Sayeeda Huq^a ; Firdausi Qadri^a ; Kaisar Talukdar^a ; Mohamed Kassam^c ; Michè ; le Delley^b ; Chloe Loiseau^b ; Ying Deng^b ; Sahar El Aidy^b ; Bernard Berger^b ; Harald Brü ; ssow^b ; ^{harald.bruessow@rdls.nestle.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Cfu ; colony forming unit ; ETEC ; enterotoxigenic E. coli ; EPEC ; enteropathogenic E. coli ; EAEC ; enteroaggregative E. coli ; M ; ColiProteus phage cocktail from Microgen ; ORS ; oral rehydration solution ; P ; placebo ; pfu ; plaque forming unit ; PT ; phage therapy ; qPCR ; quantitative polymerase chain reaction ; RCT ; randomized controlled trial ; T ; T4 phage cocktail from NRC
• 刊名：EBioMedicine
• 出版年：2016
• 出版时间：February 2016
• 年：2016
• 卷：4
• 期：Complete
• 页码：124-137
• 全文大小：2222 K

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Coliphages given orally to children with bacterial diarrhea appeared in the stool, but did not improve clinical outcome.

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In microbiologically diagnosed E. coli diarrhea, pathogen titers were close to the replication threshold of coliphages.

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Acute bacterial diarrhea displayed a marked dysbiosis with fecal streptococci that stabilized with recovery from diarrhea.

Antibiotic resistance of bacterial infections reached alarming levels. Phage therapy is a potential alternative antimicrobial. We demonstrated that two different oral phage preparations did not improve acute bacterial diarrhea in children from Bangladesh. We observed fecal excretion of the oral phage, but no major phage amplification in the gut. E. coli pathogen levels were low and the fecal microbiota showed a transient overgrowth with streptococci. Future phage trials should first verify the titer and association of the targeted pathogen with the disease.