Expression of truncated S25-229 of PEDV variant strain in Escherichia coli BL21 (DE3) resulted in inclusion body formation.
Soluble S25-229 was recovered by solubilization and refolding, followed by one-step cation exchange chromatography.
The CD spectrum of S25-229 showed the characteristic pattern of alpha helical structure.
The S25-229 showed immunogenicity and elicited strong humoral immune response in mice.