A general method to regenerate arrayed gold microelectrodes for label-free cell assay

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• 作者：Zhihui Xu^a ; Xueyan Shi^b ; Huijun Jiang^b ; Yiyan Song^a ; Liying Zhang^b ; Fangyuan Wang^b ; Shuhu Du^b ; Jin Chen^a ; ^c ; ^{okachen30@gmail.com" class="auth_mail" title="E-mail the corresponding author} ; ^{jchen@njmu.edu.cn" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Regeneration ; Arrayed gold microelectrodes ; Trypsin-digestion ; Real-time cell analyzer ; Drug cytotoxicity
• 刊名：Analytical Biochemistry
• 出版年：2017
• 出版时间：1 January 2017
• 年：2017
• 卷：516
• 期：Complete
• 页码：57-60
• 全文大小：1343 K

文摘

We developed a method to regenerate arrayed gold microelectrodes equipped for a commercial label-free cell analyzer. The regeneration process includes efficient treatment of the gold surface with trypsin (0.25%, v/v) digestion, rinsing with ethanol and deionized water and spinning steps. The proposed method ensured complete regeneration and repeated usage of gold microchips up to 4 times for the real-time electric impedance measurement of anti-cancer drug cytotoxicity.