CRISPR/Cas9 mediated knockout of the abdominal-A homeotic gene in the global pest, diamondback moth (Plutella xylostella)
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- 作者：Yuping Huanga ; b ; c ; Yazhou Chend ; Baosheng Zengd ; Yajun Wanga ; b ; c ; Anthony A. Jamese ; f ; Geoff M. Gurra ; b ; c ; g ; Guang Yanga ; b ; c ; Xijian Lina ; b ; c ; Yongping Huangd ; yphuang@sibs.ac.cn" class="auth_mail" title="E-mail the corresponding author ; Minsheng Youa ; b ; c ; msyou@iae.fjau.edu.cn" class="auth_mail" title="E-mail the corresponding author
- 关键词：Pest ; Lepidoptera ; Germline stability ; Phylogenetic analysis ; Gene mutation
- 刊名：Insect Biochemistry and Molecular Biology
- 出版年：2016
- 出版时间：August 2016
- 年：2016
- 卷：75
- 期：Complete
- 页码：98-106
- 全文大小：2559 K
文摘
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Pxabd-A has two structurally-similar splice isoforms (A and B).
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Pxabd-A exhibited varying levels of expression during development.
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CRISPR/Cas9 mediates mutated phenotypes with malformed segments, prolegs and genitals that were inherited.
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CRISPR/Cas9 mediates germline mutagenesis of the Pxabd-A genomic locus.