Fourier transform infrared spectroscopy analysis of the active components in serum of rats treated with Zuogui Pill

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• 作者：Yingli Wang ; Kaixia Xu ; Yani Li ; Qianjin Feng ; ^{xyqj728119@sina.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Zuogui Pill ; Fourier transform infrared spectroscopy ; Serum ; Fitting
• 刊名：Journal of Traditional Chinese Medical Sciences
• 出版年：2015
• 出版时间：October 2015
• 年：2015
• 卷：2
• 期：4
• 页码：264-269
• 全文大小：702 K

文摘

To investigate the active components Zuogui Pill, a typical recipe for nourishing kidney essence in the traditional Chinese medicine.

Methods

Adult male Sprague Dawley rats were treated with the traditional Chinese herbal medicine Zuogui Pill and the active components found in the serum of the animals were analyzed by Fourier transform infrared (FTIR) spectroscopy. FTIR spectra of serum samples of treated and untreated rats were analyzed and the A₂₉₆₀/A₂₉₃₁ and A₁₅₄₀/A₁₀₈₀ ratios were calculated.

Results

A₂₉₆₀/A₂₉₃₁ ratios of the serum samples collected following the administration of Zuogui Pill were significantly higher than those of the normal serum samples. FTIR data were then fitted using a Gaussian equation for wave numbers in the range of 1140–1000 cm⁻¹. A_RNA/A_DNA ratios in the serum of rats treated with Zuogui Pill were higher than those found in normal rat serum.

Conclusion

FTIR spectroscopy could be used as an analytical tool to detect the active components in serum of animals treated with Zuogui Pill.