Phosphopeptide enrichment: Development of magnetic solid phase extraction method based on polydopamine coating and Ti4+-IMAC
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- 作者：Susy Piovesana susy.piovesana@uniroma1.it" class="auth_mail" title="E-mail the corresponding author ; Anna Laura Capriotti ; annalaura.capriotti@uniroma1.it" class="auth_mail" title="E-mail the corresponding author ; Chiara Cavaliere chiara.cavaliere@uniroma1.it" class="auth_mail" title="E-mail the corresponding author ; Francesca Ferraris francesca.ferraris@uniroma1.it" class="auth_mail" title="E-mail the corresponding author ; Roberto Samperi roberto.samperi@uniroma1.it" class="auth_mail" title="E-mail the corresponding author ; Salvatore Ventura salvatore.ventura@uniroma1.it" class="auth_mail" title="E-mail the corresponding author ; Aldo Laganà ; aldo.lagana@uniroma1.it" class="auth_mail" title="E-mail the corresponding author
- 关键词：Sample preparation ; Phosphoproteomics ; Polydopamine ; Ti4+-immobilized metal-ion affinity chromatography ; Shotgun proteomics ; Magnetic solid phase extraction
- 刊名：Analytica Chimica Acta
- 出版年：2016
- 出版时间：25 February 2016
- 年：2016
- 卷：909
- 期：Complete
- 页码：67-74
- 全文大小：906 K
文摘
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Optimization of magnetic solid phase extraction method for phosphopeptides enrichment.
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Effect of loading and elution buffer composition on phosphopeptide enrichment.
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Citrate buffer is a suitable alternative eluent in Ti-IMAC.
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Method was developed employing a typical phosphoproteomic analytical workflow.
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Polydopamine allows to reduce unspecific binding during IMAC.