Identification of immunogenic proteins from ovarian tissue and recognized in larval extracts of Rhipicephalus (Boophilus) microplus, through an immunoproteomic approach
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- 作者：Patricia Berenice Ramí ; rez Rodrí ; gueza ; acineto123@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; Rodrigo Rosario Cruzb ; rockdrig@yahoo.com.mx" class="auth_mail" title="E-mail the corresponding author ; Delia Iné ; s Domí ; nguez Garcí ; ab ; deliadomgar@yahoo.com.mx" class="auth_mail" title="E-mail the corresponding author ; Rodolfo Herná ; ndez Gutié ; rreza ; rhgutierrez@ciatej.mx" class="auth_mail" title="E-mail the corresponding author ; Rodolfo Esteban Lagunes Quintanillac ; rodolfo.lagunes@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Daniel Ortuñ ; o Sahagú ; nd ; daniel.ortuno.sahagun@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Celia Gonzá ; lez Castillod ; celia_glz@hotmail.com" class="auth_mail" title="E-mail the corresponding author ; Abel Gutié ; rrez Ortegaa ; aortega@ciatej.mx" class="auth_mail" title="E-mail the corresponding author ; Sara Elisa Herrera Rodrí ; gueza ; sherrera@ciatej.mx" class="auth_mail" title="E-mail the corresponding author ; Adriana Vallejo Cardonaa ; avallejo@ciatej.mx" class="auth_mail" title="E-mail the corresponding author ; Moisé ; s Martí ; nez Velá ; zqueza ; moisesmartinezv@yahoo.com.mx" class="auth_mail" title="E-mail the corresponding author
- 关键词：Tick ; Boophilus microplus ; Ovary ; Immunogen ; Vitellogenin ; Vaccine
- 刊名：Experimental Parasitology
- 出版年：2016
- 出版时间：November 2016
- 年：2016
- 卷：170
- 期：Complete
- 页码：227-235
- 全文大小：1131 K
文摘
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A classical approach immunizing cattle with total ovarian proteins was followed.
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The detection of ovarian proteins was achieved by ELISA plates coated with proteins from larval homogenates.
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Several immunogenic proteins such as Vitellogenins and yolk Cathepsin were identified.
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Vitellogenin-1 and Vitellogenin-2 transcripts are synthesized in the ovaries of R. microplus.
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Vitellogenins represent attractive candidates for development of anti-tick vaccines.