The trehalose-based deep eutectic solvents (TCCL-DESs) were designed for protein thermostability.
The viscosities were reduced by up to 1000 times at 25 % (w/w) water addition.
TCCL3-DES and TCCL3-DES75 preserved large hydrogen bonds structures.
The thermostability of lysozyme is dramatically increased in TCCL3-DES75 by the DSC traces and CD spectrum.