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Lactose, galactose and glucose determination in naturally “lactose free” hard cheese: HPAEC-PAD method validation
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A sensitive method for lactose, galactose and glucose determination is optimized.

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Anionic chromatography with pulsed amperometric detector is applied.

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The performances are tested on long ripened PDO Grana Padano cheese.

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Results show that all the sugars are quite completely metabolized in cheese.

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