Standardization of a cytometric p24-capture bead-assay for the detection of main HIV-1 subtypes.
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- 作者：Mé ; lanie Merbaha ; b ; mmerbah@hivresearch.org" class="auth_mail" title="E-mail the corresponding author ; Sayali Onkara ; b ; sonkar@hivresearch.org" class="auth_mail" title="E-mail the corresponding author ; Jean-Charles Grivelc ; 1 ; jgrivel@sidra.org" class="auth_mail" title="E-mail the corresponding author ; Christophe Vanpouillec ; vanpouic@mail.nih.gov" class="auth_mail" title="E-mail the corresponding author ; Angé ; lique Biancottod ; biancoa@nhlbi.nih.gov" class="auth_mail" title="E-mail the corresponding author ; Lydia Bonara ; b ; lbonar@hivresearch.org" class="auth_mail" title="E-mail the corresponding author ; Eric Sanders-Buella ; b ; esanders-buell@hivresearch.org" class="auth_mail" title="E-mail the corresponding author ; Gustavo Kijaka ; b ; gkijak@hivresearch.org" class="auth_mail" title="E-mail the corresponding author ; Nelson Michaela ; nmichael@hivresearch.org" class="auth_mail" title="E-mail the corresponding author ; Merlin Robba ; b ; mrobb@hivresearch.org" class="auth_mail" title="E-mail the corresponding author ; Jerome H. Kima ; 2 ; jerome.kim@ivi.int" class="auth_mail" title="E-mail the corresponding author ; Sodsai Tovanabutraa ; b ; stovanabutra@hivresearch.org" class="auth_mail" title="E-mail the corresponding author ; Agnè ; s-Laurence Cheninea ; b ; achenine@hivresearch.org" class="auth_mail" title="E-mail the corresponding author
- 关键词：C-Ab ; capture antibody ; FI ; fluorescence intensity ; HIV-IG ; mix of human HIV immunoglobulins derived from pooled plasma of asymptomatic HIV-positive donors ; IG-beads ; beads coated with capture HIV-IG ; mAB ; monoclonal antibody ; 183-beads ; beads coated with the anti-p24 capture monoclonal antibody clone 183-H12-5C ; 39/5.4A-beads ; beads coated with the anti-p24 capture monoclonal antibody clone 39/5.4A ; 4F6-bead ; sbeads coated with the anti-p24 capture monoclonal antibody clone 4F6
- 刊名：Journal of Virological Methods
- 出版年：2016
- 出版时间：April 2016
- 年：2016
- 卷：230
- 期：Complete
- 页码：45-52
- 全文大小：606 K
文摘
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Identified a cross-reactive antibody to p24 of HIV-1 main subtypes and 2CRFs.
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Developed a p24 Luminex capture assay and tested against 56HIV-1 isolates.
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Compared the performance of the assay with other quantitative methods.
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The assay is highly sensitive with the limit of detection at 3.7 pg/ml.
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Successfully tested to detect virus production in tissue culture supernatant.