On-line preconcentration of fluorescent derivatives of catecholamines in cerebrospinal fluid using flow-gated capillary electrophoresis
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- 作者：Qiyang Zhang ; Maojun Gong ; maojun.gong@wichita.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：Flow-gated capillary electrophoresis ; Sample preconcentration ; Catecholamine ; Cerebrospinal fluid ; Laser-induced fluorescence
- 刊名：Journal of Chromatography A
- 出版年：2016
- 出版时间：10 June 2016
- 年：2016
- 卷：1450
- 期：Complete
- 页码：112-120
- 全文大小：1631 K
文摘
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A novel sample preconcentration method based on borate complexation with catecholamines was developed on the platform of flow-gated capillary electrophoresis.
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The sample mixture contained borate while the separation buffer was void of borate.
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A novel focusing mechanism for fluorescent catecholamine derivatives was proposed and experimentally studied.
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Over 100-fold signal enhancement and pico-molar detection limits for dopamine and norepinephrine were achieved with the developed method.
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The developed method was applied to the measurement of dopamine in cerebrospinal fluid.