Protective effect of Cornus walteri Wangerin leaf against UVB irradiation induced photoaging in human reconstituted skin

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• 作者：Hyun-Chul Park¹ ; ² ; ³ ; ⁴ ; ^{seung-hee@hanmail.net" class="auth_mail" title="E-mail the corresponding author} ; Taek Kyu Jung² ; ^{jungtk@saimdang.co.kr" class="auth_mail" title="E-mail the corresponding author} ; Mi Jin Kim⁵ ; ^{kmj75@saimdang.co.kr" class="auth_mail" title="E-mail the corresponding author} ; Kyung-Sup Yoon ; ¹ ; ⁶ ; ^{ksyoonjh@hanmail.net" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Anti-photoaging ; Cornus walteri Wangerin ; Matrix-metalloproteinase ; Inflammation ; Elastase ; UV-B irradiation
• 刊名：Journal of Ethnopharmacology
• 出版年：2016
• 出版时间：4 December 2016
• 年：2016
• 卷：193
• 期：Complete
• 页码：445-449
• 全文大小：991 K

文摘

Cornus walteri Wangerin has been used in oriental traditional medicine for the treatment of antidiarrheal and inflammation.

Aim of the study

The efficacy of Cornus walteri Wangerin on skin anti-photoaging was investigated.

Materials and methods

Hydrolyzed Cornus walteri Wangerin leaf was tested for the anti-photoaging effects against ultraviolet B (UVB)-induced matrix metalloproteinase (MMP)-1, pro-inflammatory cytokines using human reconstituted skin (KeraSkin™-FT) and also tested for elastase activity in vitro. The MMP-1 and pro-inflammatory cytokine levels of the extract were evaluated by enzyme-linked immunosorbent assay (ELISA).

Results

The extract of hydrolyzed Cornus walteri Wangerin leaf (CWE) had the elastase inhibitory activity (IC₅₀: 0.457 mg/mL). CWE inhibited MMP-1 expression up to 61% in comparison with the control group which was not treated using CWE, but exposed to UVB. CWE also showed an inhibitory effect on releasing pro-inflammatory cytokines (IL-6 and IL-8) in KeraSkin™-FT (30% and 57% inhibition at dose of 50 μg/mL, respectively).

Conclusion

CWE is a promising anti-photoaging agent for the treatment of UVB-induced skin.