Detection of Citrus tristeza virus by using fluorescence resonance energy transfer-based biosensor
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- 作者：Taha Roodbar Shojaeia ; tahashojaee2000@yahoo.com" class="auth_mail" title="E-mail the corresponding author ; Mohamad Amran Mohd Sallehb ; asalleh@upm.edu.my" class="auth_mail" title="E-mail the corresponding author ; Kamaruzaman Sijamc ; Raha Abdul Rahimd ; Afshin Mohsenifare ; Reza Safarnejadf ; Meisam Tabatabaeig
- 关键词：Fluorometric immunoassay ; Nanobiosensor ; Cadmium-telluride quantum dots ; Gold nanoparticles ; Bioconjugation ; Quenching
- 刊名：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
- 出版年：2016
- 出版时间：5 December 2016
- 年：2016
- 卷：169
- 期：Complete
- 页码：216-222
- 全文大小：1116 K
文摘
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Gold nanoparticles can quench QDs by using FRET phenomena.
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AuNPs/QDs FRET-based nanosensor can be used for detecting citrus tristeza virus.
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The best FRET quenching efficiency was found at 1:8.5 AuNPs/QDs ratio at about 60%.
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The accuracy of the newly developed sensor was higher than conventional detection techniques.