Deciphering the factors defining the pH-dependence of a commercial glycoside hydrolase family 8 enzyme
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- 作者：Má ; rio Barrocaa ; Gustavo Santosa ; Bjö ; rn Johanssona ; Florian Gillotinb ; Georges Fellerb ; Tony Collinsa ; tcollins@bio.uminho.pt" class="auth_mail" title="E-mail the corresponding author
- 关键词：GH8 ; glycoside hydrolase family 8 ; pXyl ; psychrophilic glycoside hydrolase family 8 xylanase (UniProtKB entry Q8RJN8) ; MOPS ; 3-(N-morpholino)propanesulfonic acid ; MES ; 2-(N-morpholino)ethanesulfonic acid ; TAPS ; N-[Tris(hydroxymethyl)methyl]-3-aminopropanesulfonic acid ; CHES ; N-cyclohexyl-2-aminoethanesulfonic acid ; CAPS ; N-cyclohexyl-3-aminopropanesulfonic acid
- 刊名：Enzyme and Microbial Technology
- 出版年：2017
- 出版时间：January 2017
- 年：2017
- 卷：96
- 期：Complete
- 页码：163-169
- 全文大小：967 K
文摘
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An in-depth analysis of the pH dependence of a commercial xylanase is described.
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Diverse reversible and irreversible processes play varying roles at distinct pHs.
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Precipitation plays a major role at both acidic and basic pHs.
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Different molecular determinants are proposed to be involved at different pHs.