PPARγ activation alters fatty acid composition in adipose triglyceride, in addition to proliferation of small adipocytes, in insulin resistant high-fat fed rats

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• 作者：Daisuke Sato^a ; ^{d_sato@yz.yamagata-u.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Kanako Oda^a ; Masataka Kusunoki^b ; ^{info@tonyo.jp" class="auth_mail" title="E-mail the corresponding author} ; Atsuyoshi Nishina^c ; ^{nishina.atsuyoshi@nihon-u.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Kazuaki Takahashi^d ; ^{k-takahashi@yone.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Zhonggang Feng^e ; ^{zhgfeng@yz.yamagata-u.ac.jp" class="auth_mail" title="E-mail the corresponding author} ; Kazuhiko Tsutsumi^f ; ^{kazu4786@chive.ocn.ne.jp" class="auth_mail" title="E-mail the corresponding author} ; Takao Nakamura^a ; ^{task-n@yz.yamagata-u.ac.jp" class="auth_mail" title="E-mail the corresponding author}
• 关键词：Peroxisome proliferator-activated receptor γ ; Fatty acid composition ; Adipocyte size ; Insulin resistance ; Thiazolidinedione
• 刊名：European Journal of Pharmacology
• 出版年：2016
• 出版时间：15 February 2016
• 年：2016
• 卷：773
• 期：Complete
• 页码：71-77
• 全文大小：1197 K

文摘

It was reported that adipocyte size is potentially correlated in part to amount of long chain polyunsaturated fatty acids (PUFAs) and insulin resistance because several long chain PUFAs can be ligands of peroxisome proliferator-activated receptors (PPARs). In our previous study, marked reduction of PUFAs was observed in insulin-resistant high-fat fed rats, which may indicate that PUFAs are consumed to improve insulin resistance. Although PPARγ agonist, well known as an insulin sensitizer, proliferates small adipocytes, the effects of PPARγ agonist on FA composition in adipose tissue have not been clarified yet. In the present study, we administered pioglitazone, a PPARγ agonist, to high-fat fed rats, and measured their FA composition of triglyceride fraction in adipose tissue and adipocyte diameters in pioglitazone-treated (PIO) and non-treated (control) rats. Insulin sensitivity was obtained with hyperinsulinemic euglycemic clamp. Average adipocyte diameter in the PIO group were smaller than that in the control one without change in tissue weight. In monounsaturated FAs (MUFAs), 14:1n-5, 16:1n-7, and 18:1n-9 contents in the PIO group were lower than those, respectively, in the control group. In contrast, 22:6n-3, 20:3n-6, 20:4n-6, and 22:4n-6 contents in the PIO group were higher than those, respectively, in the control group. Insulin sensitivity was higher in the PIO group than in the control one. These findings suggest that PPARγ activation lowered MUFAs whereas suppressed most of C20 or C22 PUFAs reduction, and that the change of fatty acid composition may be relevant with increase in small adipocytes.