KDM4C, a H3K9me3 Histone Demethylase, is Involved in the Maintenance of Human ESCC-Initiating Cells by Epigenetically Enhancing SOX2 Expression

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• 作者：Xiang Yuan^* ; ² ; ^{yuanxiangdrive@163.com" class="auth_mail" title="E-mail the corresponding author} ; Jinyu Kong^* ; ^{kongjinyu@126.com" class="auth_mail" title="E-mail the corresponding author} ; Zhikun Ma^* ; ^{mazhikun1225@163.com" class="auth_mail" title="E-mail the corresponding author} ; Na Li^* ; ^{linatec83@163.com" class="auth_mail" title="E-mail the corresponding author} ; Ruinuo Jia^* ; ^{ruinuojia78@163.com" class="auth_mail" title="E-mail the corresponding author} ; Yiwen Liu^* ; ^{liuyiwen615810@163.com" class="auth_mail" title="E-mail the corresponding author} ; Fuyou Zhou^§ ; ; Qimin Zhan^&Dagger ; ; ^{qmzhan59@163.com" class="auth_mail" title="E-mail the corresponding author} ; Gang Liu^* ; ^&dagger ; ; ^{liuga@karmanos.org" class="auth_mail" title="E-mail the corresponding author} ; Shegan Gao^* ; ^{gsgvicepresident@163.com" class="auth_mail" title="E-mail the corresponding author}
• 关键词：ESCC ; Esophageal squamous cell carcinoma ; TICs ; Tumor-initiating cells ; KDM4C ; Lysine (K)-specific demethylase 4C
• 刊名：Neoplasia
• 出版年：2016
• 出版时间：October 2016
• 年：2016
• 卷：18
• 期：10
• 页码：594-609
• 全文大小：3144 K

文摘

Our studies investigating the existence of tumor-initiating cell (TIC) populations in human esophageal squamous cell carcinoma (ESCC) had identified a subpopulation of cells isolated from ESCC patient-derived tumor specimens marked by an ALDH^bri+ phenotype bear stem cell-like features. Importantly, KDM4C, a histone demethylase was enhanced in ALDH^bri+ subpopulation, suggesting that strategies interfering with KDM4C may be able to target these putative TICs. In the present study, by genetic and chemical means, we demonstrated that, KDM4C blockade selectively decreased the ESCC ALDH^bri+ TICs population in vitro and specifically targeted the TICs in ALDH^bri+-derived xenograft, retarding engraftment. Subsequent studies of the KDM4C functional network identified a subset of pluripotency-associated genes (PAGs) and aldehyde dehydrogenase family members to be preferentially down-regulated in KDM4C inhibited ALDH^bri+ TICs. We further supported a model in which KDM4C maintains permissive histone modifications with a low level of H3K9 methylation at the promoters of several PAGs. Moreover, ectopic expression of SOX2 restored KDM4C inhibition-dependent ALDH^bri+ TIC properties. We further confirmed these findings by showing that the cytoplasmic and nuclear KDM4C staining increased with adverse pathologic phenotypes and poor patient survival. Such staining pattern of intracellular KDM4C appeared to overlap with the expression of SOX2 and ALDH1. Collectively, our findings provided the insights into the development of novel therapeutic strategies based on the inhibition of KDM4C pathway for the eliminating of ESCC TIC compartment.