Countercurrent chromatography separation of saponins by skeleton type from Ampelozizyphus amazonicus for off-line ultra-high-performance liquid chromatography/high resolution accurate mass spectrometry analysis and characterisation

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• 作者：Fabiana de Souza Figueiredo^a ; Rita Celano^b ; Danila de Sousa Silva^c ; Fernanda das Neves Costa^a ; Peter Hewitson^d ; Svetlana Ignatova^d ; Anna Lisa Piccinelli^b ; Luca Rastrelli^b ; Suzana Guimarã ; es Leitã ; o^c ; Gilda Guimarã ; es Leitã ; o^a ; ^{ggleitao@yahoo.com.br} ; ^{ggleitao@nppn.ufrj.br}
• 关键词：Ampelozizyphus amazonicus ; Rhamnaceae ; Saponin skeleton type ; Triterpene saponins ; Countercurrent chromatography ; HPLC&ndash ; MS
• 刊名：Journal of Chromatography A
• 出版年：2017
• 出版时间：20 January 2017
• 年：2017
• 卷：1481
• 期：Complete
• 页码：92-100
• 全文大小：581 K
• 卷排序：1481

文摘

Methodologies were developed for separation and characterisation of butanol extract from Ampelozizyphus amazonicus bark. CCC separation of saponins by skeleton type from butanol extract produced oleane type and dammarane type groups. The characterisation of saponins was done by UHPLC–MS. Unusual aglycones saponins as C30 and C31 keto-dammarane were characterized by UHPLC–MS.