Cloning, characterization and expression of a novel haplotype cry2A-type gene from Bacillus thuringiensis strain SWK1, native to Himalayan valley Kashmir
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- 作者：A.L. Reyaz ; P. Indra Arulselvi ; iarulselvibiotech@gmail.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Bacillus thuringiensis ; Cloning ; Protein expression ; SDS-PAGE ; Bioassay ; cry2Al1
- 刊名：Journal of Invertebrate Pathology
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：136
- 期：Complete
- 页码：1-6
- 全文大小：736 K
文摘
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A new cry2A gene was cloned and expressed from a promising indigenous Bacillus thuringiensis SWK1 strain.
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B. thuringiensis δ-endotoxin Nomenclature Committee designated this new cry gene as cry2Al1.
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Expressed Cry2Al1 protein showed toxicity to Spodoptera litura and Helicoverpa armigera.