Development of a loop-mediated isothermal amplification method for rapid detection of streptococcal pyrogenic exotoxin B
详细信息 查看全文
- 作者：Cuiming Cao ; Fang Zhang ; Mingyu Ji ; Fengyan Pei ; Xiujie Fan ; Hong Shen ; Qingxi Wang ; Weihua Yang ; whyxp@163.com" class="auth_mail" title="E-mail the corresponding author ; Yunshan Wang ; sdjnwys@163.com" class="auth_mail" title="E-mail the corresponding author
- 关键词：Streptococcus pyogenes ; speB gene ; Loop-mediated isothermal amplification (LAMP) ; Rapid detection ; Primers
- 刊名：Toxicon
- 出版年：2016
- 出版时间：July 2016
- 年：2016
- 卷：117
- 期：Complete
- 页码：53-58
- 全文大小：570 K
文摘
- •
SpeB is among the important toxins produced by S. pyogenes, it exists in almost all S. pyogenes strains.
- •
The LAMP method is not affected by impurities in the reaction system.
- •
The positive results were easily detected by the colour changes with the naked eye.
- •
Detecting the speB gene by the LAMP assay is highly specific and sensitive, is simple to perform and cost-effective.
- •
This method is Valuable and feasible for the rapid diagnosis of S. pyogenes infection in developing countries.