Cultivated ginseng inhibits 2,4-dinitrochlorobenzene-induced atopic dermatitis-like skin lesions in NC/Nga mice and TNF-¦Á/IFN-¦Ã-induced TARC activation in HaCaT cells

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• 作者：Jae Ho Choi^a ; ¹ ; Sun Woo Jin^a ; ¹ ; Bong Hwan Park^a ; Hyung Gyun Kim^a ; Tilak Khanal^a ; Hwa Jeong Han^a ; Yong Pil Hwang^a ; ^b ; Jun Min Choi^c ; Young Chul Chung^c ; Sang Kyu Hwang^d ; Tae Cheon Jeong^e ; ^{hgjeong@cnu.ac.kr} ; Hye Gwang Jeong^a ; ^{taecheon@ynu.ac.kr}
• 关键词：Cultivated ginseng ; Atopic dermatitis ; IgE ; TARC ; NF-¦ÊB
• 刊名：Food and Chemical Toxicology
• 出版年：2013
• 出版时间：June, 2013
• 年：2013
• 卷：56
• 期：Complete
• 页码：195-203
• 全文大小：816 K

文摘

Ginseng contains many bioactive constituents, including various ginsenosides that are believed to have anti-allergic, anti-oxidant, and immunostimulatory activities; however, its effects on atopic dermatitis (AD) remain unclear. In the current study, we hypothesized that cultivated ginseng (CG) would inhibit 2,4-dinitrochlorobenzene (DNCB)-induced AD-like skin lesions in NC/Nga mice by regulating the T helper (Th)1/Th2 balance. Also, CG inhibits TNF-¦Á/IFN-¦Ã-induced thymus- and activation-regulated chemokine (TARC) expression through nuclear factor-kappa B (NF-¦ÊB)-dependent signaling in HaCaT cells. CG ameliorated DNCB-induced dermatitis severity, serum levels of IgE and TARC, and mRNA expression of TARC, TNF-¦Á, IFN-¦Ã, IL-4, IL-5, and IL-13 in mice. Histopathological examination showed reduced thickness of the epidermis/dermis and dermal infiltration of inflammatory cells in the ears. Furthermore, CG suppressed the TNF-¦Á/IFN-¦Ã-induced mRNA expression of TARC in HaCaT cells. CG inhibited TNF-¦Á/IFN-¦Ã-induced NF-¦ÊB activation. These results suggest that CG inhibited the development of the AD-like skin symptoms by modulating Th1 and Th2 responses in the skin lesions in mice and TARC expression by suppressing TNF-¦Á/IFN-¦Ã-induced NF-¦ÊB activation in keratinocytes, and so may be a useful tool in the therapy of AD-like skin symptoms.