Development and characterization of a human embryonic stem cell-derived 3D neural tissue model for neurotoxicity testing

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• 作者：J Sandströ ; m^a ; ¹ ; E Eggermann^b ; ¹ ; I Charvet^b ; ¹ ; A Roux^b ; N Toni^c ; C Greggio^a ; A Broyer^a ; F Monnet-Tschudi^a ; ¹ ; L Stoppini^b ; ¹
• 关键词：Human embryonic stem cells ; 3D cultures ; Neurons ; Astrocytes ; Oligodendrocytes ; Spontaneous electrical activity ; Neurotoxicity testing
• 刊名：Toxicology in Vitro
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：38
• 期：Complete
• 页码：124-135
• 全文大小：2034 K
• 卷排序：38

文摘

Free-floating 3D cultures from hESC acquire a high degree of differentiation. Neurons, astrocytes and oligodendrocytes are organized in a histotypic manner. Electrophysiological measurements showed different firing patterns. Acute treatment with known neurotoxicants upregulated HMOX and GFAP expression. These 3D cultures could be a useful model for developmental neurotoxicity testing.