A method for identification of HIV gp140 binding memory B cells in human blood

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• 作者：Johannes F. Scheid ; Hugo Mouquet ; Niklas Feldhahn ; Bruce D. Walker ; Florencia Pereyra ; Emily Cutrell ; Michael S. Seaman ; John R. Mascola ; Richard T. Wyatt ; Hedda Wardemann ; Michel C. Nussenzweig
• 关键词：HIV ; Memory B cells ; gp140
• 刊名：Journal of Immunological Methods
• 出版年：2009
• 出版时间：15 April 2009
• 年：2009
• 卷：343
• 期：2
• 页码：65-67
• 全文大小：214 K

文摘

Antibodies to HIV are potentially important reagents for basic and clinical studies. Historically, these reagents have been produced by random cloning of heavy and light chains in phage display libraries [Burton, D.R., Barbas, C.F. III, Persson, M.A.A., Koenig, S., Chanock, R.M., and Lerner, R.A., (1991), A large array of human monoclonal antibodies to type 1 immnodeficiency virus from combinatorial libraries of asymptomatic seropositive individuals. Proc. Natl. Acad. Sci. U. S. A. 88, 10134–10137.] and electrofusion techniques [Buchacher, A., Predl, R., Tauer, C., Purtscher, M., Gruber, G., Heider, R., Steindl, F., Trkola, A., Jungbauer, A., and Katinger, H., (1992), Human monoclonal antibodies against gp41 and gp120 as potential agent for passive immunization. Vaccines 92, 191–195]. Here we describe a method to identify and potentially enrich human memory B cells from HIV infected patients that show serum titers of neutralizing antibodies. When biotinylated gp140 is used to stain peripheral blood mononuclear cells it identifies a distinct population of gp140 binding B cells by flow cytometry.