Qualitative and quantitative analysis of PMN/T-cell interactions by InFlow and super-resolution microscopy

详细信息    查看全文

• 作者：Emre Balta¹ ; Julian Stopp¹ ; Laura Castelletti ; Henning Kirchgessner ; Yvonne Samstag ; Guido H. Wabnitz ; ^{guido.wabnitz@immu.uni-heidelberg.de}
• 关键词：Neutrophils ; Immune synapse ; Actin cytoskeleton ; Transdifferentiation ; Imaging flow cytometry
• 刊名：Methods
• 出版年：2017
• 出版时间：1 January 2017
• 年：2017
• 卷：112
• 期：Complete
• 页码：25-38
• 全文大小：3273 K
• 卷排序：112

文摘

Transdifferentiated human PMN form immune synapses with T-cells. A work flow to analyse PMN/T-cell interactions is provided. F-actin is more dynamic at the T-cell site of the immune synapse. Capabilities and limitations of InFlow microscopy are discussed.