Specificity enhancement towards phenolic substrate by immobilization of laccase on surface plasmon resonance sensor chip
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- 作者：Safoura Jabbari ; Bahareh Dabirmanesh ; Khosro Khajeh ; khajeh@modares.ac.ir" class="auth_mail" title="E-mail the corresponding author
- 关键词：Laccase ; Surface plasmon resonance ; Phenolic compounds ; Substrate specificity ; Covalent immobilization
- 刊名：Journal of Molecular Catalysis B: Enzymatic
- 出版年：2015
- 出版时间：November 2015
- 年：2015
- 卷：121
- 期：Complete
- 页码：32-36
- 全文大小：1501 K
文摘
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Laccases are polyphenol oxidases that exhibit broad substrate specificity.
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A laccase from Bacillus sp. HR03 was immobilized on the carboxymethyl dextran chip.
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The immobilization of laccase altered its substrate specificity toward phenolic substrate.
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Atomic force microscopy revealed a uniform distribution of laccase over the sensor surface.
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The results could lead to the utility of laccase in developing a specific phenolic biosensor.