Epithelial Cell Adhesion Molecule Expression in Canine Tumours

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• 作者：D.H. Thamm^&lowast ; ; ^&dagger ; ; ^§ ; ; ^{dthamm@colostate.edu} ; D.F. Hayes^‖ ; T. Meuten^&Dagger ; ; T. Laver^&lowast ; ; D.G. Thomas^‖
• 关键词：carcinoma ; dog ; epithelial cell adhesion molecule ; immunohistochemistry
• 刊名：Journal of Comparative Pathology
• 出版年：2016
• 出版时间：November 2016
• 年：2016
• 卷：155
• 期：4
• 页码：299-304
• 全文大小：1027 K
• 卷排序：155

文摘

Epithelial cell adhesion molecule (EpCAM) is expressed in most human normal and neoplastic tissues of epithelial derivation and may have an association with tumour cell aggressiveness, a stem cell-like phenotype and clinical outcome. Antibody-based strategies for the targeting and capture of EpCAM-expressing tumour cells are showing promise, both as diagnostic tools and potential therapies. The aim of this study was to assess EpCAM expression in canine tumours. EpCAM expression was assessed in tumour cell lines via gene expression profiling and in formalin-fixed and paraffin wax-embedded tissues from canine carcinomas representing various anatomical sites by immunohistochemistry. EpCAM mRNA expression was higher in cell lines from carcinomas than those derived from sarcomas or haemopoietic tumours. EpCAM was expressed by >2/3 of tumour cells in 71% of canine carcinomas evaluated, irrespective of histotype, with the exception of carcinomas of the adrenal gland. Canine sarcomas and haemopoietic tumours were uniformly negative. Most canine carcinomas express EpCAM and so could be suitable for the study of EpCAM-directed diagnostics and therapeutics.