Treatment of ovariectomized rats with 17¦Â-estradiol increases hepatic delta-6 desaturase enzyme expression and docosahexaenoic acid levels in hepatic and plasma phospholipids

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• 作者：Alex P. Kitson ; Kristin A. Marks ; Brittany Shaw ; David M. Mutch ; Ken D. Stark
• 关键词：Omega-3 polyunsaturated fatty acids ; Sex hormones ; Ovariectomy ; Delta-6 desaturase ; Docosahexaenoic acid
• 刊名：Prostaglandins, Leukotrienes and Essential Fatty Acids (PLEFA)
• 出版年：2013
• 出版时间：August, 2013
• 年：2013
• 卷：89
• 期：2-3
• 页码：81-88
• 全文大小：492 K

文摘

Higher docosahexaenoic acid (DHA) in females compared with males suggests ovarian hormones increase DHA production. Eight-week old rats were ovariectomized or sham operated (SHAM), and ovariectomized rats were treated with implanted pellets providing 17¦Â-estradiol (OVX+E), progesterone (OVX+P), both (OVX+PE) or neither (OVX) for 14 days. Immunoblot and fatty acid analysis were performed on all samples, and microarray analysis was performed on OVX and SHAM groups. Increased ¦¤6-desaturase in OVX relative to SHAM was observed by microarray (12 % higher) and immunoblot (31 % higher). OVX+E and OVX+PE rats had 39 % and 42 % higher ¦¤6-desaturase content, respectively, compared with OVX. OVX+E and OVX+PE also increased phospholipid DHA concentrations in liver (increase of 34 % and 40 % , respectively) and plasma (increase of 70 % and 74 % , respectively) relative to OVX. Progesterone exerted no effect on ¦¤6-desaturase or DHA. These results indicate that 17¦Â-estradiol increases DHA through increased ¦¤6-desaturase, possibly explaining sex differences in DHA.