In vivo tissue engineered bone versus autologous bone: stability and structure

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• 作者：R.M. Zimmerer¹ ; ^{zimmerer.ruediger@mh-hannover.de} ; P. Jehn¹ ; H. Kokemü ; ller¹ ; R. Abedian² ; M. Lalk³ ; F. Tavassol¹ ; N.-C. Gellrich¹ ; S. Spalthoff¹
• 关键词：bioartificial bone ; axial vascularization ; bone engineering ; custom-made transplants ; heterotopic bone growth ; flap prefabrication ; maxillofacial reconstruction ; vascularized bone transplants ; biomechanical tests ; Young's modulus ; elastic modulus ; micro-CT
• 刊名：International Journal of Oral and Maxillofacial Surgery
• 出版年：2017
• 出版时间：March 2017
• 年：2017
• 卷：46
• 期：3
• 页码：385-393
• 全文大小：2863 K
• 卷排序：46

文摘

This pilot study investigated the biomechanical properties of prefabricated, vascularized bioartificial bone grafts, which may provide an alternative bone source for the restoration of segmental osseous defects. Vascularized bioartificial bone grafts comprise an artificial customized scaffold made of beta-tricalcium phosphate. Bone formation along the prefabricated scaffold is induced by autogenous cancellous bone. Vascularization of the bone graft is provided by the host's vascular system. Within 6 months, a mammalian bioreactor (sheep were used in the present study) creates heterotopic vascularized bioartificial bone grafts of a predetermined anatomical shape, which can be harvested for reconstructing osseous defects. The bioartificial bone grafts in this study contained up to 25% bone tissue, as shown by histomorphometric analysis and computed tomography. Moreover, unconfined compression tests revealed that the constructs had mechanical characteristics similar to those of ovine cancellous bone. Therefore, this method could be applied to generate vascularized prefabricated bone substitutes for critical-size defects.