Rapid microwave-assisted synthesis of sub-30 nm lipid nanoparticles

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• 作者：Stuart S. Dunn^a ; ^b ; ^{ssdunn@unc.edu} ; Denis R. Beckford Vera^a ; ^b ; ^{denisrbv@email.unc.edu} ; S. Rahima Benhabbour^c ; ^{benhabs@email.unc.edu} ; Matthew C. Parrott^a ; ^b ; ^d ; ^{parrotmc@email.unc.edu}
• 关键词：NPs ; Nanoparticles ; Tm ; melting temperature ; BTM ; BTL ; or BTMy ; Brij 78 ; vitamin E TPGS ; and Miglyol 812 ; triLaurin ; or triMyristin ; delta-10 (Δ10) ; the point at which particle size increased by 10% ; BTM65/125 ; BTM NPs fabricated at 65 ° ; C or 125 ° ; C ; BTL65/125 ; BTL NPs fabricated at 65 ° ; C or 125 ° ; C ; Dz ; Z-average diameter ; PDI ; polydispersity index
• 刊名：Journal of Colloid and Interface Science
• 出版年：2017
• 出版时间：15 February 2017
• 年：2017
• 卷：488
• 期：Complete
• 页码：240-245
• 全文大小：550 K
• 卷排序：488

文摘

Accessing the phase inversion temperature by microwave heating may enable the rapid synthesis of small lipid nanoparticles.ExperimentsNanoparticle formulations consisted of surfactants Brij 78 and Vitamin E TPGS, and trilaurin, trimyristin, or miglyol 812 as nanoparticle lipid cores. Each formulation was placed in water and heated by microwave irradiation at temperatures ranging from 65 °C to 245 °C. We observed a phase inversion temperature (PIT) for these formulations based on a dramatic decrease in particle Z-average diameters. Subsequently, nanoparticles were manufactured above and below the PIT and studied for (a) stability toward dilution, (b) stability over time, (c) fabrication as a function of reaction time, and (d) transmittance of lipid nanoparticle dispersions.FindingsLipid-based nanoparticles with distinct sizes down to 20–30 nm and low polydispersity could be attained by a simple, one-pot microwave synthesis. This was carried out by accessing the phase inversion temperature using microwave heating. Nanoparticles could be synthesized in just one minute and select compositions demonstrated high stability. The notable stability of these particles may be explained by the combination of van der Waals interactions and steric repulsion. 20–30 nm nanoparticles were found to be optically transparent.