Hydroxylation of lithocholic acid by selected actinobacteria and filamentous fungi

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• 作者：V.V. Kollerov^a ; D. Monti^b ; ^{daniela.monti@icrm.cnr.it} ; N.O. Deshcherevskaya^a ; T.G. Lobastova^a ; E.E. Ferrandi^b ; A. Larovere^b ; S.A. Gulevskaya^a ; S. Riva^b ; M.V. Donova^a ; ^{donova@ibpm.pushchino.ru}
• 关键词：Bile acids ; Bioconversion ; Lithocholic acid ; Ursodeoxycholic acid ; 7¦Â-Hydroxylation ; Filamentous fungi
• 刊名：Steroids
• 出版年：2013
• 出版时间：March, 2013
• 年：2013
• 卷：78
• 期：3
• 页码：370-378
• 全文大小：608 K

文摘

Selected actinobacteria and filamentous fungi of different taxonomy were screened for the ability to carry out regio- and stereospecific hydroxylation of lithocholic acid (LCA) at position 7¦Â. The production of ursodeoxycholic acid (UDCA) was for the first time shown for the fungal strains of Bipolaris, Gibberella, Cunninghamella and Curvularia, as well as for isolated actinobacterial strains of Pseudonocardia, Saccharothrix, Amycolatopsis, Lentzea, Saccharopolyspora and Nocardia genera. Along with UDCA, chenodeoxycholic (CDCA), deoxycholic (DCA), cholic (CA), 7-ketodeoxycholic and 3-ketodeoxycholic acids were detected amongst the metabolites by some strains.

A strain of Gibberella zeae VKM F-2600 expressed high level of 7¦Â-hydroxylating activity towards LCA. Under optimized conditions, the yield of UDCA reached 90 % at 1 g/L of LCA and up to 60 % at a 8-fold increased substrate loading. The accumulation of the major by-product, 3-keto UDCA, was limited by using selected biotransformation media.