The effect of unpredictable chronic mild stress on depressive-like behavior and on hippocampal A₁ and striatal A_2A adenosine receptors

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• 作者：Leonardo M. Crema^a ; ^b ; Let&iacute ; cia F. Pettenuzzo^a ; ^c ; Michele Schlabitz^a ; Luisa Diehl^a ; ^b ; Juliana Hoppe^a ; ^c ; R&eacute ; gis Mestriner^a ; ^d ; Daniela Laureano^a ; ^b ; Christianne Salbego^a ; ^c ; Carla Dalmaz^a ; ^b ; ^c ; Deusa Vendite^a ; ^c ; ^{vendite.ez@terra.com.br}
• 关键词：Chronic stress ; Depression ; Hippocampus ; Striatum ; Adenosine ; A1 receptor ; A2A receptor ; Binding ; Western blotting
• 刊名：Physiology and Behavior
• 出版年：2013
• 出版时间：17 January, 2013
• 年：2013
• 卷：109
• 期：Complete
• 页码：1-7
• 全文大小：519 K

文摘

This study examined the effects of two chronic stress regimens upon depressive-like behavior, A₁ and A_2A adenosine receptor binding and immunocontent. Male rats were subjected to unpredictable chronic mild stress (UCMS) or to chronic restraint stress (CRS) for 40 days. Subsequently, depressive-like behaviors (forced swimming and consumption of sucrose) were evaluated, and A₁ adenosine or A_2A adenosine receptors were examined in the hippocampus or striatum, respectively. UCMS animals demonstrated depressive-related behaviors (decrease in sucrose consumption and increased immobility in the forced swimming test). This group also presented increased A₁ adenosine receptor binding and immunoreactivity in hippocampus, as well as increased striatal A_2A adenosine receptor binding in the striatum, without alteration in immunoreactivity. Conversely, the chronic restraint stress group displayed only an increase in A₁ adenosine receptor binding and no alteration in the other parameters evaluated. We suggest that the alteration in adenosine receptors, particularly the upregulation of striatal A_2A adenosine receptors following UCMS, could be associated with depressive-related behavior.