Effects of polysaccharides from Pholiota nameko on maturation of murine bone marrow-derived dendritic cells

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• 作者：Haiping Li ; Lizeng Liu ; Yongqing Tao ; Pei Zhao ; Fengling Wang ; Lihua Huai ; Dexian Zhi ; Jiangmei Liu ; Guoliang Li ; Chunlan Dang ; Yufeng Xu
• 关键词：AFM ; atomic force microscopy ; APCs ; antigen presenting cells ; BMDCs ; bone marrow dendritic cells ; DCs ; dendritic cells ; ELISA ; enzyme linked immunosorbent assay ; FCM ; flow cytometry ; imBMDCs ; immature BMDCs ; LPS ; lipopolysaccharides ; MACS ; magnetic activated cell sorting ; MLN ; mesenteric lymph nodes ; PNPS-1 ; Pholiota nameko polysaccharides ; RBC ; red blood cell ; SEM ; scanning electronic microscopy ; PAMPs ; pathogen-associated molecular patterns ; TNF ; tumor necrosis factor.
• 刊名：International Journal of Biological Macromolecules
• 出版年：February, 2014
• 年：2014
• 卷：63
• 期：Complete
• 页码：188-197
• 全文大小：2242 K

文摘

This paper studied some structure characters of the Pholiota nameko polysaccharides (PNPS-1), including morphology under SEM and AFM, also the effects of PNPS-1 on the maturation of bone marrow dendritic cells (BMDCs) via concrete changes both inside and outside BMDCs. These impacts on BMDCs were assessed with use of inverted phase contrast microscope for morphology, flow cytometry for key surface molecules, mixed lymphocyte reaction (MLR) for allogeneic T cells proliferation, and bio-assay and enzyme linked immunosorbent assay (ELISA) for cytokine production. We found that PNPS-1 could inhibit phenotypic maturation as evidenced by decreasing expression of CD11c, CD40, CD80, CD83, CD86, and I-A/I-E. Functional maturation inhibition was further confirmed by decreased naive T cell stimulatory activity of BMDCs. Finally, PNPS-1 also stimulated production of more cytokine IL-10 and less IL-12 and TNF-伪. These data indicated that PNPS-1 could markedly inhibit the maturation of BMDCs and had potential significant down-regulation immunity.