Identification of conserved miRNAs and their putative target genes in Podophyllum hexandrum (Himalayan Mayapple)
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- 作者：Soumi Biswas soumibiswas.23690@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Saptarshi Hazra sapta.hazra@gmail.com" class="auth_mail" title="E-mail the corresponding author ; Sharmila Chattopadhyay ; sharmila@iicb.res.in" class="auth_mail" title="E-mail the corresponding author
- 关键词：A ; adenosine ; BLASTX ; Basic Local Alignment Search Tool X ; C ; cytidine ; cDNA ; DNA complementary to RNA ; DCL1 ; Dicer-like protein 1 ; dNTP ; deoxyribonucleoside triphosphate ; ds ; double stranded ; DTT ; dithiothreitol ; EC ; enzyme commission ; EST ; expressed sequence tags ; G ; guanosine ; GO ; gene ontology ; GSS ; genomic survey sequences ; HIV ; human immunodeficiency virus ; miRNA ; micro ribonucleic acid ; μl ; microliter ; MFE ; minimal folding free energy ; MFEI ; minimal folding free energy index ; ng ; nanogram
- 刊名：Plant Gene
- 出版年：2016
- 出版时间：June 2016
- 年：2016
- 卷：6
- 期：Complete
- 页码：82-89
- 全文大小：651 K
文摘
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Identification of miRNAs in P.hexandrum — an endangered medicinal plant.
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Prediction of target genes of P.hexandrum miRNAs and their functional annotation.
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Validation of P.hexandrum miRNAs through stem-loop qRT-PCR.