Differential scanning fluorimetry based assessments of the thermal and kinetic stability of peptide-MHC complexes
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- 作者：Lance M. Hellmana ; Liusong Yinb ; Yuan Wanga ; Sydney J. Blevinsa ; Timothy P. Rileya ; Orrin S. Beldena ; Timothy T. Spearc ; Michael I. Nishimurac ; Lawrence J. Sternb ; d ; Brian M. Bakera ; brian-baker@nd.edu" class="auth_mail" title="E-mail the corresponding author
- 关键词：DSF ; differential scanning fluorimetry ; Tm ; melting temperature ; CD ; circular dichroism
- 刊名：Journal of Immunological Methods
- 出版年：2016
- 出版时间：May 2016
- 年：2016
- 卷：432
- 期：Complete
- 页码：95-101
- 全文大小：804 K
文摘
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The thermal stability of peptide/MHC complexes correlates with peptide affinity.
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Differential scanning fluorimetry permits rapid assessment of peptide/MHC stability.
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Substantially lower volume requirements of DSF provides for high throughput.
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DSF also allows for measurements of peptide/MHC kinetic stability/dissociation rates.
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Complexities not detectable with other binding assays can be observed with DSF.
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