Reduction of CRKL expression in patients with partial DiGeorge syndrome is associated with impairment of T-cell functions

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• 作者：Mauro Giacomelli ; PhD^a ; ^b ; ^&lowast ; ; Rajesh Kumar ; PhD^a ; ^b ; ^&lowast ; ; Annarosa Soresina ; MD^a ; Nicola Tamassia ; PhD^c ; Tiziana Lorenzini ; MD^a ; Daniele Moratto ; PhD^a ; Sara Gasperini ; PhD^c ; Marco Cassatella ; MD^c ; Alessandro Plebani ; MD ; PhD^a ; Vassilios Lougaris ; MD ; PhD^a ; Raffaele Badolato ; MD ; PhD^a ; ^{raffaele.badolato@unibs.it" class="auth_mail" title="E-mail the corresponding author}
• 关键词：CRKL ; DiGeorge syndrome ; c-Fos ; proliferation ; T-cell receptor activation ; IL-2 ; signal transducer and activator of transcription 5
• 刊名：Journal of Allergy and Clinical Immunology
• 出版年：2016
• 出版时间：July 2016
• 年：2016
• 卷：138
• 期：1
• 页码：229-240.e3
• 全文大小：941 K

文摘

Partial DiGeorge syndrome (pDGS) is caused by deletion of the 22q11.2 region. Within this region lies CrK-like (CRKL), a gene encoding an adapter protein belonging to the Crk family that is involved in the signaling cascade of IL-2, stromal cell–derived factor 1α, and type I interferon. Although recurrent infections can be observed in patients with deletion of chromosome 22 syndrome, the immune pathogenesis of this condition is yet not fully understood.

Objective

We aimed to investigate the role of CRKL in T-cell functional responses in patients affected with pDGS.

Methods

Protein expression levels and phosphorylation of CRKL were evaluated in patients with pDGS. T-cell functional assays in vitro and gene-silencing experiments were also performed.

Results

CRKL protein expression, as well as its phosphorylation, were reduced in all patients with pDGS, especially on IL-2 stimulation. Moreover, T cells presented impaired proliferation and reduced IL-2 production on anti-CD3/CD28 stimulation and decreased c-Fos expression. Finally, CRKL silencing in Jurkat T cells resulted in impaired T-cell proliferation and reduced c-Fos expression.

Conclusions

The impaired T-cell proliferation and reduction of CRKL, phosphorylated CRKL, and c-Fos levels suggest a possible role of CRKL in functional deficiencies of T cells in patients with pDGS.