Nucleotide Sequence of a cDNA Clone Encoding aCaenorhabditis elegansHomolog of Mammalian Alkyl-Dihydroxyacetonephosphate Synthase: Evolutionary Switching of Peroxisomal Targeting Signals

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• 作者：de Vet ; Edwin C. J. M. ; Prinsen ; Hubertus C. M. T. ; van den Bosch ; Henk
• 刊名：Biochemical and Biophysical Research Communications
• 出版年：1998
• 出版时间：January 14, 1998
• 年：1998
• 卷：242
• 期：2
• 页码：277-281
• 全文大小：271 K

文摘

The nucleotide sequence is reported of a cDNA clone encoding aCaenorhabditis eleganshomolog of guinea pig and human alkyl-dihydroxyacetonephosphate synthase. The open reading frame encodes a protein of 597 amino acids which shows extensive homology with the mammalian enzymes (52 % identical and about 76 % similar in the overlapping region). In contrast to the mammalian enzymes, which carry a consensus peroxisomal targeting signal type 2 in a cleavable N-terminal presequence, thisCaenorhabditis eleganshomolog carries a consensus peroxisomal targeting signal type 1 (CKL) at its C-terminus. Expression of this protein in anin vitrotranscription/translation system yielded a 65 kDa protein. Recombinantaenorhabditis elegansalkyl-DHAP synthase expressed in the yeastPichia pastoriswas enzymatically active.