Three-dimensional gold nanoparticles/prussian blue-poly(3,4-ethylenedioxythiophene) nanocomposite as novel redox matrix for label-free electrochemical immunoassay of carcinoembryonic antigen

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• 作者：Taotao Yang^a ; ^bAuthor Vitae ; Yansha Gao^aAuthor Vitae ; Zhen Liu^aAuthor Vitae ; Jingkun Xu ; Dr.^a ; ^{xujingkun@tsinghua.org.cn" class="auth_mail" title="E-mail the corresponding author} (Prof.)Author Vitae ; Limin Lu ; Dr.^b ; ^{lulimin816@hotmail.com" class="auth_mail" title="E-mail the corresponding author}Author Vitae ; Yongfang Yu^bAuthor Vitae
• 关键词：Gold nanoparticles ; Prussian blue ; 3D PEDOT ; Carcinoembryonic antigen ; Label-free immunosensor
• 刊名：Sensors & Actuators: B. Chemical
• 出版年：2017
• 出版时间：February 2017
• 年：2017
• 卷：239
• 期：Complete
• 页码：76-84
• 全文大小：2696 K

文摘

We present a novel label-free electrochemical immunosensor for detecting carcinoembryonic antigen (CEA) based on three-dimensional (3D) gold nanoparticles/prussian blue-poly(3,4-ethylenedioxythiophene) (AuNPs/PB-PEDOT) nanocomposite, which was firstly synthesized by a simple redox reaction between the PB precursors and EDOT in an aqueous solution, followed by the electrochemical reduction of HAuCl₄. The 3D nanocomposite not only possessed large surface area and favorable microenvironment, but also exhibited remarkable conductivity, stability and excellent biocompatibility. In addition, PB showed excellent redox properties. Then AuNPs/PB-PEDOT was used as both electron mediators and 3D matrices in the fabrication of immunosensor. FT-IR spectra were employed to confirm the formation of PB, PEDOT and PB-PEDOT. Significantly, the AuNPs/PB-PEDOT exhibited a 3D and hierarchically porous nanostructure, while the PB-PEDOT showed core-shell structure. The AuNPs/PB-PEDOT modified immunosensor showed good linearity with the concentration of CEA ranging from 0.05 to 40 ng mL⁻¹, and the detection limit was 0.01 ng mL⁻¹. Moreover, the prepared electrode displayed good selectivity, high stability and good repeatability, and showed great potential for application in real sample analysis.