Biomarkers of liver fibrosis detecting with electrochemical immunosensor on clinical serum

详细信息    查看全文

• 作者：Yao Yao^a ; ¹Author Vitae ; Jianfeng Bao^b ; ¹Author Vitae ; Yanli Lu^aAuthor Vitae ; Diming Zhang^aAuthor Vitae ; Senbiao Luo^cAuthor Vitae ; Xing Cheng^aAuthor Vitae ; Qian Zhang^aAuthor Vitae ; Shuang Li^aAuthor Vitae ; Qingjun Liu^a ; ^{qjliu@zju.edu.cn" class="auth_mail" title="E-mail the corresponding author}Author Vitae
• 关键词：Liver fibrosis ; Biosensor ; Serum biomarker ; Human transforming growth factor beta 1 ; Hyaluronate acid ; Impedance
• 刊名：Sensors & Actuators: B. Chemical
• 出版年：2016
• 出版时间：January 2016
• 年：2016
• 卷：222
• 期：Complete
• 页码：127-132
• 全文大小：1494 K

文摘

Diagnosing hepatic fibrosis at an early stage with sensitive and specific monitoring approach is crucial for patient therapeutics and survival. In this study, an electrochemical immunosensor was established to detect representative biomarkers of liver fibrosis, such as hyaluronate acid (HA) and transforming growth factor beta 1 (TGF尾1). Through a self-assembled monolayer of polyethylene glycol (PEG), antibodies against HA and TGF尾1 were successfully immobilized on interdigitated electrodes. It produced a robust and sensitive membrane by improving the uniformity, density, and distribution of the antibodies for the biomarkers. Based on impedance sensing, HA and TGF尾1 were sensitively detected in the ranges of 1–1000 ng/ml. The detection limits of HA and TGF尾1 reached 0.586 ng/ml and 0.570 ng/ml, respectively. In addition, for the detection of clinical serum samples, the results were in excellent agreement with the tests of HA, type III pre-collagen (PCIII), IV collagen (IV-C), and laminin (LN) that conducted by radioimmunoassay for liver fibrosis. The research indicated that the approach provided a valuable, universal, and label-free strategy in evaluating liver fibrosis and other chronic diseases for point-of-care diagnostics.