Flow-injection chemiluminescence determination of dihydralazine sulfate in serum using luminol and diperiodatocuprate (III) system

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• 作者：Chunyan Yang ; Zhujun Zhang ; Jinli Wang
• 关键词：Chemiluminescence ; Diperiodatocuprate ; Dihydralazine sulfate ; Serum
• 刊名：Spectrochimica Acta Part A: Molecular and Biomolecular Spectroscopy
• 出版年：2010
• 出版时间：January 2010
• 年：2010
• 卷：75
• 期：1
• 页码：77-82
• 全文大小：258 K

文摘

A novel flow-injection chemiluminescence (CL) method for the determination of dihydralazine sulfate (DHZS) is described. The method is based on the reaction of luminol and diperiodatocuprate (K₂[Cu(H₂IO₆)(OH)₂], DPC) in alkaline medium to emit CL, which is greatly enhanced by DHZS. The possible CL mechanism was first proposed based on the kinetic characteristic, CL spectrum and UV spectra. The optimum condition for the CL reaction was in detail studied using flow-injection system. The experiments indicated that under optimum condition, the CL intensity was linearly related to the concentration of DHZS in the range of 7.0 × 10⁻⁹ to 8.6 × 10⁻⁷ g mL⁻¹ with a detection limit (3σ) of 2.1 × 10⁻⁹ g mL⁻¹. The proposed method had good reproducibility with the relative standard deviation 3.1 % (n = 7) for 5.2 × 10⁻⁸ g mL⁻¹ of DHZS. This method has the advantages of simple operation, fast response and high sensitivity. The special advantage of the system is that very low concentration of luminol can react with DPC catalyzed by DHZS to get excellent experiment results. And CL cannot be observed nearly when luminol with same concentration reacts with other oxidants, so luminol–DPC system has higher selectivity than other luminol CL systems. The method has been successfully applied to determine DHZS in serum.