Human liver cytosolic sulfotransferase 2A1-dependent dehydroepiandrosterone sulfation assay by ultra-high performance liquid chromatography-tandem mass spectrometry
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- 作者：Sumit Bansala ; Aik Jiang Laua ; b ; aikjiang.lau@nus.edu.sg" class="auth_mail" title="E-mail the corresponding author
- 关键词：DHEA ; dehydroepiandrosterone ; DHEA-S ; dehydroepiandrosterone sulfate ; DMSO ; dimethyl sulfoxide ; DTT ; DL-dithiothreitol ; HPLC&ndash ; UV ; high-performance liquid chromatography&ndash ; ultraviolet spectroscopy ; LC&ndash ; MS/MS ; liquid chromatography&ndash ; tandem mass spectrometry ; MgCl2 ; magnesium chloride ; MRM ; multiple reaction monitoring ; PAP ; 3&prime ; -phosphoadenosine 5&prime ; -phosphate ; PAPS ; 3&prime ; -phosphoadenosine 5&prime ; -phosphosulfate ; SULT ; sulfotransferase ; SULT2A1 ; sulfotransferase 2A1
- 刊名：Journal of Pharmaceutical and Biomedical Analysis
- 出版年：2016
- 出版时间：20 February 2016
- 年：2016
- 卷：120
- 期：Complete
- 页码：261-269
- 全文大小：1491 K
文摘
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Human liver cytosolic SULT2A1-dependent DHEA sulfation assay by UPLC–MS/MS.
Endogenous chemicals in human liver cytosol did not affect the assay.
Reducing agents did not affect DHEA sulfation by human liver cytosol.
DHEA sulfation was analyzed as a function of enzyme amount and incubation time.
Safer, easier, and faster than existing radiometric-based methods.