DIXDC1 co-localizes and interacts with γ-tubulin in HEK293 cells

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• 作者：Yanrui Wu ; Xiaotang Jing ; Xin Ma ; Yan Wu ; Xuefeng Ding ; Wenhong Fan ; Ming Fan
• 关键词：DIXDC1 ; γ ; -tubulin ; Centrosome ; Co-localization
• 刊名：Cell Biology International
• 出版年：2009
• 出版时间：June 2009
• 年：2009
• 卷：33
• 期：6
• 页码：697-701
• 全文大小：348 K

文摘

DIXDC1 is a Dishevelled-Axin (DIX) domain-containing protein involved in neural development and Wnt signaling pathway. Besides the DIX domain, DIXDC1 also contains a coiled-coil domain (MTH domain), which is a common feature of centrosomal proteins. We have demonstrated that exogenously expressed GFP-tag fused DIXDC1 co-localize with γ-tubulin both at interphase and mitotic phase in HEK293 cells. By immunostaining with anti-DIXDC1 and anti-γ-tubulin antibody, endogenous DIXDC1 was also co-localized with γ-tubulin at the centrosomes in HEK293 cells. We confirmed this interaction of DIXDC1 with γ-tubulin by co-immunoprecipitation. The findings suggest that DIXDC1 might play an important role in chromosome segregation and cell cycle regulation.