The effects of particulate ambient air pollution on the murine umbilical cord and its vessels: A quantitative morphological and immunohistochemical study

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• 作者：Mariana Matera Veras^a ; ^c ; ^{verasine@usp.br} ; Rosane Maria Guimarã ; es-Silva^b ; Elia Garcia Caldini^b ; ^c ; Paulo H.N. Saldiva^a ; ^c ; Marisa Dolhnikoff^a ; ^c ; Terry M. Mayhew^d
• 关键词：Particulate air pollution ; Umbilical cord ; Stereology ; Immunohistochemistry ; Endothelin receptors ; Isoprostane ; Collagen
• 刊名：Reproductive Toxicology
• 出版年：2012
• 出版时间：December, 2012
• 年：2012
• 卷：34
• 期：4
• 页码：598-606
• 全文大小：1145 K

文摘

Previous studies have shown that particulate matter (PM) compromise birth weight and placental morphology. We hypothesized that exposing mice to ambient PM would affect umbilical cord (UC) morphology. To test this, mice were kept in paired open-top exposure chambers at the same location and ambient conditions but, in one chamber, the air was filtered (F) and, in the other, it was not (NF). UCs were analysed stereologically and by immunohistochemistry to localize isoprostane and endothelin receptors. The cords of mice from NF chambers were smaller in volume due to loss of mucoid connective tissue and decrease in volume of collagen. These structural changes and in umbilical vessels were associated with greater volumes of regions immunostained for isoprostane, ET_AR and ET_BR. Findings indicate that the adverse effects of PM on birth weight may be mediated in part by alterations in UC structure or imbalances in the endogenous regulators of vascular tone and oxidative stress.