TPC Proteins Are Phosphoinositide- Activated Sodium-Selective Ion Channels in Endosomes and Lysosomes

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• 作者：Xiang Wang ; Xiaoli Zhang ; Xian-ping Dong ; Mohammad Samie ; Xinran Li ; Xiping Cheng ; Andrew Goschka ; Dongbiao Shen ; Y ; ong Zhou ; Janice Harlow ; Michael?X. Zhu ; David?E. Clapham ; Dejian Ren ; Haoxing Xu
• 刊名：Cell
• 出版年：2012
• 出版时间：12 October, 2012
• 年：2012
• 卷：151
• 期：2
• 页码：372-383
• 全文大小：6534 K

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Summary

Mammalian two-pore channel proteins (TPC1, TPC2; TPCN1, TPCN2) encode ion channels in intracellular endosomes and lysosomes and were proposed to mediate endolysosomal calcium release triggered by the second messenger, nicotinic acid adenine dinucleotide phosphate (NAADP). By directly recording TPCs in endolysosomes from wild-type and TPC?double-knockout mice, here we show that, in contrast to previous conclusions, TPCs are in fact sodium-selective channels activated by PI(3,5)P₂ and are not activated by NAADP. Moreover, the primary endolysosomal ion is Na⁺, not K⁺, as had been previously assumed. These findings suggest that the organellar membrane potential may undergo large regulatory changes and may explain the specificity of PI(3,5)P₂ in regulating the fusogenic potential of intracellular organelles.