Cloning, expression and antiviral bioactivity of Red-crowned Crane interferon-伪
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- 作者：Li Tiana ; tianli_1988@yahoo.com" class="auth_mail ; Panpan Zhaoa ; zpp13796033057@163.com" class="auth_mail ; Bo Mab ; maboLHT@163.com" class="auth_mail ; Guangyang Guoa ; guowanyang2009@163.com" class="auth_mail ; Ying Suna ; sunyingdongzi@163.com" class="auth_mail ; Mingwei Xinga ; xingmingweinefu@sina.com" class="auth_mail
- 关键词：IFN-伪 ; interferon-伪 ; crIFN-伪 ; Red-crowned Crane interferon-伪 ; ORF ; open reading frame ; VSV ; vesicular stomatitis virus ; UTR ; untranslated regions ; 5&prime ; UTR ; 5&prime ; untranslated regions ; 3&prime ; UTR ; 3&prime ; untranslated regions ; CEF ; chicken fibroblasts ; FITC-goat-anti-rabbit IgG ; fluorescein isothiocyanate-conjugated goat anti-rabbit IgG
- 刊名：Gene
- 出版年：1 July 2014
- 年：2014
- 卷：544
- 期：1
- 页码：49-55
- 全文大小：2202 K
文摘
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We provide the IFN-伪 gene sequence of Red-crowned Crane for the first time.
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The crIFN-伪 expressed in E. coli could inhibit the replication of VSV on CEF.
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The antiviral activities were abrogated by rabbit anti-crIFN-伪 antibodies in vitro.
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CrIFN-伪 could be expressed in CEF and was mainly located in the cytoplasm.