Purification and characterisation of dsRNA using ion pair reverse phase chromatography and mass spectrometry

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• 作者：Alison O. Nwokeoji^a ; An-Wen Kung^a ; Peter M. Kilby^b ; David E. Portwood^b ; Mark J. Dickman^a ; ^{m.dickman@sheffield.ac.uk}
• 关键词：Double stranded RNA purification ; RNase mass mapping ; Ion pair reverse phase HPLC ; Mass spectrometry
• 刊名：Journal of Chromatography A
• 出版年：2017
• 出版时间：10 February 2017
• 年：2017
• 卷：1484
• 期：Complete
• 页码：14-25
• 全文大小：2170 K
• 卷排序：1484

文摘

rapid purification of dsRNA in a single step protocol. high throughput purification and analysis of a wide range of dsRNAs. developed IP RP HPLC for the rapid, high resolution analysis of the dsRNA. developed a novel method utilising RNase T1 for RNase mass mapping of dsRNA.