Forces guiding staphylococcal adhesion

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• 作者：Philippe Herman-Bausier^a ; Cé ; cile Formosa-Dague^a ; Cé ; cile Feuillie^a ; Claire Valotteau^a ; Yves F. Dufrê ; ne^a ; ^b ; ^{Yves.Dufrene@uclouvain.be}
• 关键词：Atomic force microscopy ; Molecular forces ; Bacterial adhesion ; Biofilms ; Nosocomial infections ; Staphylococcus aureus ; Staphylococcus epidermidis
• 刊名：Journal of Structural Biology
• 出版年：2017
• 出版时间：January 2017
• 年：2017
• 卷：197
• 期：1
• 页码：65-69
• 全文大小：835 K
• 卷排序：197

文摘

Staphylococcus epidermidis and Staphylococcus aureus are two important nosocomial pathogens that form biofilms on indwelling medical devices. Biofilm infections are difficult to fight as cells within the biofilm show increased resistance to antibiotics. Our understanding of the molecular interactions driving bacterial adhesion, the first stage of biofilm formation, has long been hampered by the paucity of appropriate force-measuring techniques. In this minireview, we discuss how atomic force microscopy techniques have enabled to shed light on the molecular forces at play during staphylococcal adhesion. Specific highlights include the study of the binding mechanisms of adhesion molecules by means of single-molecule force spectroscopy, the measurement of the forces involved in whole cell interactions using single-cell force spectroscopy, and the probing of the nanobiophysical properties of living bacteria via multiparametric imaging. Collectively, these findings emphasize the notion that force and function are tightly connected in staphylococcal adhesion.