Hepatoprotective effects of Vernonia amygdalina (astereaceae) in rats treated with carbon tetrachloride

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• 作者：Omolola A. Adesanoye ; Ebenezer O. Farombi
• 关键词：Vernonia amygdalina ; Carbon tetrachloride (CCl₄) ; Antioxidants ; Hepatotoxicity ; Free radicals ; Reactive oxygen species (ROS) ; Liver
• 刊名：Experimental and Toxicologic Pathology
• 出版年：2010
• 出版时间：March 2010
• 年：2010
• 卷：62
• 期：2
• 页码：197-206
• 全文大小：376 K

文摘

The possible modulatory effect of methanolic extract of Vernonia amygdalina (MEVA), a plant widely consumed in the tropics and used locally in the treatment of fever, jaundice, stomach disorders and diabetes on the toxicity of CCl₄, was investigated in male rats.

Oral administration of CCl₄ at a dose of 1.2 g/kg body weight 3 times a week for 3 weeks significantly induced marked hepatic injury as revealed by increased activity of the serum enzymes ALT, AST, SALP and γ-GT. Methanolic extract of V. amygdalina administered 5 times a week for 2 weeks before CCl₄ treatment at 250 and 500 mg/kg doses of the extract ameliorated the increase in the activities of these enzymes. Likewise the methanolic extract of V. amygdalina reduced the CCl₄-induced increase in the concentrations of cholesterol, triglyceride and phospholipid by 37.8 % , 30.6 % and 8.5 % , respectively, and a reduction in the cholesterol/phospholipids ratio. These parameters were however increased at 750 mg/kg extract pretreatment. CCl₄-induced lipid peroxidation was likewise attenuated by 57.2 % at 500 mg/kg dose of the methanolic extract of V. amygdalina. Similarly, administration of the extract increased the activities of the antioxidant enzymes: superoxide dismutase, glutathione S-transferase and reduced glutathione concentration significantly at 500 mg/kg (P<0.05) and catalase activity at 500–1000 mg/kg doses. These results suggest that methanolic extract of V. amygdalina leaves posseses protective effect against CCl₄-induced hepatotoxicity by the antioxidant mechanism of action.